This directory contains: - jetty.zip version 7.1.4 of jetty with configuration that allows easy deployment of Plasmapper (port 8082) - PlasMapper_download.tar.gz version 2.0 of PlasMapper downloaded from http://wishart.biology.ualberta.ca/PlasMapper. - PlasMapper_dist.zip compressed distribution directory created in step 5 of the following instruction - YeastLab-common_enzymes.txt list of common enzymes used in Yeast Lab - YeastLab-PlasMapper_dist.zip (TODO) distribution with common enzymes changed to the ones used in Yeast Lab - example.gb exmple GB file that can be used for testing after installation is complete *Installation instructions for version 2.0* 1. Unpack PlasMapper_download.tar.gz into your workspace. 2. In order to use the project in eclipse perform the following steps: - Create a new project in eclipse using PlasMapper as source directory - Fix build path: -- remove /tmp/tomcat/common/lib/servlet-api.jar -- add /libraries/jetty/servlet-api-2.5.jar - [optional] change settings (can be done later, when deploying the application) -- properties file: ca.ualberta.xdong.plasMapper.annotate.plasMapConfiguration_en_CA.properties -- change '/home/tomcat/webapps/PlasMapper' to the path where PlasMapper will be deployed -- 'plasMapRoot' leads to an external directory, where PlasMapper will create tmp directory for its output 3. in build.xml change values of properties: - - 4. [optional] change list of common enzymes in ca.ualberta.xdong.plasMapper.annotate.PlasmidCleavageSiteAnnotate.java (for Yeast Lab see YeasLab-common_enzymes.txt) 5. run ant install 6. copy dist/webapps/PlasMapper to webapps of the application server (make sure plasMapConfiguration_en_CA.properties are properly configured) Additionally there needs to be blastall installed on the machine running PlasMapper. Latest version can be downloaded from ftp://ftp.ncbi.nih.gov/blast/executables/release/LATEST. Go to http://:8080/PlasMapper/ after deployment to check that everything is working. You should be able to generate a PNG for 'example.gb' file from this directory: - select the GB file - change the Image Size from 850x750 to 1200x1000 - click on Graphic Map button (below DNA sequence text area)