/* * Copyright 2010 ETH Zuerich, CISD * * Licensed under the Apache License, Version 2.0 (the "License"); * you may not use this file except in compliance with the License. * You may obtain a copy of the License at * * http://www.apache.org/licenses/LICENSE-2.0 * * Unless required by applicable law or agreed to in writing, software * distributed under the License is distributed on an "AS IS" BASIS, * WITHOUT WARRANTIES OR CONDITIONS OF ANY KIND, either express or implied. * See the License for the specific language governing permissions and * limitations under the License. */ import java.io.BufferedOutputStream; import java.io.BufferedReader; import java.io.File; import java.io.FileOutputStream; import java.io.FileReader; import java.io.IOException; import java.io.OutputStream; import java.util.ArrayList; import java.util.Arrays; import java.util.Collection; import java.util.HashMap; import java.util.Iterator; import java.util.List; import java.util.Map; import ch.systemsx.cisd.openbis.dss.client.api.v1.IDataSetDss; import ch.systemsx.cisd.openbis.dss.generic.shared.api.v1.NewDataSetMetadataDTO; import ch.systemsx.cisd.openbis.generic.client.cli.Login; import ch.systemsx.cisd.openbis.plugin.screening.client.api.v1.IScreeningOpenbisServiceFacade; import ch.systemsx.cisd.openbis.plugin.screening.client.api.v1.IScreeningOpenbisServiceFacadeFactory; import ch.systemsx.cisd.openbis.plugin.screening.client.api.v1.ScreeningOpenbisServiceFacade.IImageOutputStreamProvider; import ch.systemsx.cisd.openbis.plugin.screening.client.api.v1.ScreeningOpenbisServiceFacadeFactory; import ch.systemsx.cisd.openbis.plugin.screening.shared.api.v1.dto.ExperimentIdentifier; import ch.systemsx.cisd.openbis.plugin.screening.shared.api.v1.dto.FeatureVector; import ch.systemsx.cisd.openbis.plugin.screening.shared.api.v1.dto.FeatureVectorDataset; import ch.systemsx.cisd.openbis.plugin.screening.shared.api.v1.dto.FeatureVectorDatasetReference; import ch.systemsx.cisd.openbis.plugin.screening.shared.api.v1.dto.FeatureVectorWithDescription; import ch.systemsx.cisd.openbis.plugin.screening.shared.api.v1.dto.Geometry; import ch.systemsx.cisd.openbis.plugin.screening.shared.api.v1.dto.ImageDatasetMetadata; import ch.systemsx.cisd.openbis.plugin.screening.shared.api.v1.dto.ImageDatasetReference; import ch.systemsx.cisd.openbis.plugin.screening.shared.api.v1.dto.MaterialIdentifier; import ch.systemsx.cisd.openbis.plugin.screening.shared.api.v1.dto.MaterialTypeIdentifier; import ch.systemsx.cisd.openbis.plugin.screening.shared.api.v1.dto.Plate; import ch.systemsx.cisd.openbis.plugin.screening.shared.api.v1.dto.PlateIdentifier; import ch.systemsx.cisd.openbis.plugin.screening.shared.api.v1.dto.PlateImageReference; import ch.systemsx.cisd.openbis.plugin.screening.shared.api.v1.dto.PlateWellMaterialMapping; import ch.systemsx.cisd.openbis.plugin.screening.shared.api.v1.dto.WellIdentifier; import ch.systemsx.cisd.openbis.plugin.screening.shared.api.v1.dto.WellPosition; /** * Simple Matlab interface for openBIS for Screening. It is meant to be used in one Matlab session * at a time, i.e. it is not multi-threading safe. *

* While written in Java, the API is idiomatic for Matlab, i.e. values are returned as * multi-dimensional arrays. For the get... and load... methods the first * index will contain the actual data, while the second index will contain per-row annotations. For * getFeatureMatrix, the third index contains per-column annotations. This allows * simple access with Matlab's slicing operator, see doc of e.g. {@link #getFeatureMatrix(String)}. *

* A typical Matlab session looks like: * *

 * % Add the API jar file to the classpath
 * javaaddpath('/home/brinn/matlab/openbis_screening_api-batteries_included.jar')
 * % Login to server
 * OpenBISScreeningML.login('user', 'secret', 'https://www.infectome.org')
 * 
 * % ...perform calls on the server...
 * 
 * % Logout to close the session on the server
 * OpenBISScreeningML.logout()
 * 
* * Note: using this login your password will end up in the Matlab command history. An alternative * that avoids this is to call the {@link ch.systemsx.cisd.openbis.generic.client.cli.Login} class. * Logging in on the console will grant this class access to the openBIS server. *

* To learn the API one needs to understand three basic notions: code, augmented code and perm id. * Space, project, experiment, plate and well have their own code, which is unique only in * the context of the parent.
* That's why one needs augmented code to point e.g. to one experiment, because two different * projects can have experiments with the same code.
* Such an augmented code for experiment has a form of "/space-code/project-code/experiment-code".
* For plate it has a form of "/space-code/plate-code" (note that plate code is unique on the space * level).
* The drawback of an augmented code is that it's not persistent. If someone e.g. moves the * experiment from one space to the other augmented code of the experiment becomes invalid. That is * why experiments, plates and datasets have perm id (permament identifier) which never * change and allow to refer to them with one "magic" identifier, e.g. 20110516124520378-737166. *

* * @author Bernd Rinn */ public class OpenBISScreeningML { private static interface ITileNumberIterable extends Iterable { public void setMaximumNumberOfTiles(int numberOfTiles); public int getMaximumNumberOfTiles(); } private static final class ImageReferenceAndFile { private final PlateImageReference imageReference; private final File imageFile; private BufferedOutputStream outputStream; ImageReferenceAndFile(PlateImageReference imageReference, File imageFile) { this.imageReference = imageReference; this.imageFile = imageFile; } public PlateImageReference getImageReference() { return imageReference; } public File getImageFile() { return imageFile; } public OutputStream open() throws IOException { if (outputStream == null) { outputStream = new BufferedOutputStream(new FileOutputStream(imageFile)); } return outputStream; } public void close() throws IOException { if (outputStream != null) { outputStream.close(); } outputStream = null; } } static final String DATASETS_FOLDER = "openbis_datasets"; private static File temporarySessionDir; private static Map loadedImages; static IScreeningOpenbisServiceFacadeFactory facadeFactory = ScreeningOpenbisServiceFacadeFactory.INSTANCE; private static IScreeningOpenbisServiceFacade openbis = null; private static List experiments = null; private static List plates = null; private static Map> experimentToPlateMap = new HashMap>(); private static Map experimentCodeToExperimentMap = new HashMap(); private static Map plateCodeToPlateMap = new HashMap(); private OpenBISScreeningML() { // Not to be constructed. } // // Versioning // /** * The version of the API. */ public static final String VERSION = "1"; /** * The required version ("major.minor") of the screening API on the openBIS application server. */ public static final String REQUIRES_OPENBIS_AS_API = "1.7"; /** * The required version ("major.minor") of the screening API on the openBIS datastore server. */ public static final String REQUIRES_OPENBIS_DSS_API = "1.1"; private static File dataSetsDir; /** * Root temporary directory for data sets and images. By default java.io.tmpdir is * used. */ static File tempDir = new File(System.getProperty("java.io.tmpdir")); static final String TEMP_DIR_PREFIX = "openbis_"; static final String TEMP_DIR_POSTFIX = "_temp_dir"; // // Authentication methods // /** * Login to the openBIS server given as url. *

* Matlab example: * *

     * OpenBISScreeningML.login('user', 'secret', 'https://www.infectome.org')
     * 
* * @param user The user id on the server * @param password The password on the server * @param url The URL, e.g. https://www.infectome.org */ public static void login(String user, String password, String url) { IScreeningOpenbisServiceFacade facade = facadeFactory.tryToCreate(user, password, url); if (facade == null) { throw new RuntimeException("Login failed."); } init(facade); } private static void init(IScreeningOpenbisServiceFacade openBisFacade) { openbis = openBisFacade; dataSetsDir = new File(tempDir, DATASETS_FOLDER); if (dataSetsDir.isDirectory() == false && dataSetsDir.mkdirs() == false) { throw new RuntimeException("Couldn't create a data set directory."); } temporarySessionDir = new File(tempDir, TEMP_DIR_PREFIX + System.currentTimeMillis() / 1000 + TEMP_DIR_POSTFIX); if (temporarySessionDir.mkdirs() == false) { throw new RuntimeException("Couldn't create a temporary directory."); } loadedImages = new HashMap(); experiments = openbis.listExperiments(); experimentCodeToExperimentMap.clear(); for (ExperimentIdentifier e : experiments) { experimentCodeToExperimentMap.put(e.getAugmentedCode(), e); } plates = openbis.listPlates(); plateCodeToPlateMap.clear(); experimentToPlateMap.clear(); for (Plate p : plates) { final String plateCode = p.getAugmentedCode(); plateCodeToPlateMap.put(plateCode, p); final String experimentCode = p.getExperimentIdentifier().getAugmentedCode(); List experimentPlates = experimentToPlateMap.get(experimentCode); if (experimentPlates == null) { experimentPlates = new ArrayList(); experimentToPlateMap.put(experimentCode, experimentPlates); } experimentPlates.add(p); } } /** * Logs out and closes the session on the server. *

* Matlab example: * *

     * OpenBISScreeningML.logout()
     * 
*/ public static void logout() { if (openbis == null) { return; } openbis.logout(); if (Login.OPENBIS_TOKEN_FILE.exists()) { Login.OPENBIS_TOKEN_FILE.delete(); } delete(temporarySessionDir); openbis = null; } private static void delete(File file) { if (file.isDirectory()) { File[] files = file.listFiles(); for (File child : files) { delete(child); } } file.delete(); } // // Information methods // /** * Lists all experiment. *

* Matlab example: * *

     * % Get the experiments
     * exps = OpenBISScreeningML.listExperiments();
     * % How many experiments do we have?
     * length(exps)
     * % Get all information about experiment 3
     * exp3 = exps(3,:)
     * % Get the perm ids for all experiments
     * permids = exps(:,2)
     * 
* * @return Each row contains information about one experiment: *

* { experiment augmented code, experiment perm id, experiment space code, * experiment project code, experiment code } */ public static Object[][] listExperiments() { checkLoggedIn(); final Object[][] result = new Object[experiments.size()][5]; for (int i = 0; i < experiments.size(); ++i) { final Object[] annotations = new Object[] { experiments.get(i).getAugmentedCode(), experiments.get(i).getPermId(), experiments.get(i).getSpaceCode(), experiments.get(i).getProjectCode(), experiments.get(i).getExperimentCode() }; System.arraycopy(annotations, 0, result[i], 0, annotations.length); } return result; } /** * Lists all plates. *

* Matlab example: * *

     * % Get the plates
     * plates = OpenBISScreeningML.listPlates();
     * % How many plates do we have?
     * length(plates)
     * % Get all information about plate 2
     * plate2 = plates(2,:)
     * % Get the simple plate codes for all plates
     * codes = plates(:,4)
     * 
* * @return Each row contains information about one plate: *

* { plate augmented code, plate perm id, plate space code, plate code, * experiment augmented code, experiment perm id, experiment space code, * experiment project code, experiment code } */ public static Object[][] listPlates() { checkLoggedIn(); return listPlates(plates); } /** * Lists the plates of experiment. *

* Matlab example: * *

     * % Get the plates of experiment MYEXP in project PROJ of space SPACE
     * plates = OpenBISScreeningML.listPlates('/SPACE/PROJ/MYEXP');
     * % How many plates do we have?
     * length(plates)
     * % Get all information about plate 2
     * plate2 = plates(2,:)
     * % Get the augmented plate codes for all plates
     * acodes = plates(:,1)
     * 
* * @param experiment The augmented code of the experiment to list the plates for * @return Each row contains information about one plate: *

* { plate augmented code, plate perm id, plate space code, plate code, * experiment augmented code, experiment perm id, experiment space code, * experiment project code, experiment code } */ public static Object[][] listPlates(String experiment) { checkLoggedIn(); final List experimentPlates = experimentToPlateMap.get(experiment); if (experimentPlates == null) { throw new RuntimeException("No experiment with that code found."); } return listPlates(experimentPlates); } private static Object[][] listPlates(final List list) { final Object[][] result = new Object[list.size()][9]; for (int i = 0; i < list.size(); ++i) { final Object[] annotations = new Object[] { list.get(i).getAugmentedCode(), plates.get(i).getPermId(), list.get(i).tryGetSpaceCode(), plates.get(i).getPlateCode(), list.get(i).getExperimentIdentifier().getAugmentedCode(), list.get(i).getExperimentIdentifier().getPermId(), list.get(i).getExperimentIdentifier().getSpaceCode(), list.get(i).getExperimentIdentifier().getProjectCode(), list.get(i).getExperimentIdentifier().getExperimentCode(), }; System.arraycopy(annotations, 0, result[i], 0, annotations.length); } return result; } /** * Returns the properties of specified well for specified plate. *

* Matlab example: * *

     * % Get properties for well A03 of plate P005 in space SPACE
     * properties = OpenBISScreeningML.getWellProperties('/SPACE/P005', 1, 3)
     * % Get property type code of first property
     * properties(1,1)
     * % Get property value of first property
     * properties(1,2)
     * 
* * @param augmentedPlateCode The augmented plate code * @param row The row in the plate to get the well properties for * @param column The column in the plate to get the well properties for * @return A two dimensional array where the first column contains the property codes and the * second column the corresponding property values. */ public static Object[][] getWellProperties(String augmentedPlateCode, int row, int column) { checkLoggedIn(); WellPosition wellPosition = new WellPosition(row, column); WellIdentifier wellIdentifier = getWell(augmentedPlateCode, wellPosition); List> list = new ArrayList>(openbis.getWellProperties(wellIdentifier) .entrySet()); Object[][] result = new Object[list.size()][2]; for (int i = 0; i < list.size(); i++) { result[i] = new Object[] { list.get(i).getKey(), list.get(i).getValue() }; } return result; } /** * Updates properties of specified well for specified plate. *

* Matlab example: * *

     * % Updates properties DESCRIPTION and NUMBER for well A03 of plate P005 in space SPACE
     * properties = {'DESCRIPTION' 'hello example'; 'NUMBER' 3.14}
     * OpenBISScreeningML.updateWellProperties('/SPACE/P005', 1, 3, properties)
     * 
* * @param augmentedPlateCode The augmented plate code * @param row The row in the plate to get the well properties for * @param column The column in the plate to get the well properties for * @param properties A two dimensional array where the first column contains the property codes * and the second column the corresponding property values. */ public static void updateWellProperties(String augmentedPlateCode, int row, int column, Object[][] properties) { checkLoggedIn(); WellPosition wellPosition = new WellPosition(row, column); WellIdentifier wellIdentifier = getWell(augmentedPlateCode, wellPosition); openbis.updateWellProperties(wellIdentifier, createMap(properties)); } private static WellIdentifier getWell(String augmentedPlateCode, WellPosition wellPosition) { Plate plate = getPlate(augmentedPlateCode); List wells = openbis.listPlateWells(plate); for (WellIdentifier wellIdentifier : wells) { if (wellIdentifier.getWellPosition().equals(wellPosition)) { return wellIdentifier; } } throw new RuntimeException("Plate '" + augmentedPlateCode + "' has no well at " + wellPosition + "."); } /** * Fetches metadata of the image datasets for the specified plate. *

* Matlab example: * *

     * % Get the metadata of image datasets of plate P005 from space SPACE
     * imagesMetadata = OpenBISScreeningML.getImagesMetadata('/SPACE/P005');
     * % How many image datasets do we have? Usually there will be just one.
     * length(imagesMetadata)
     * % What is the number of tiles in the first image dataset?
     * imagesMetadata(1, 3)
     * 
* * @param augmentedPlateCode The augmented plate code. * @return { images width, images height, number of tiles in the well, * number of tiles rows, number of tiles columns, number of plate rows, number of plate columns } * . */ public static Object[][] getImagesMetadata(String augmentedPlateCode) { checkLoggedIn(); final Plate plateId = getPlate(augmentedPlateCode); final List imageDatasets = listRawImageDatasets(plateId); final List metaList = openbis.listImageMetadata(imageDatasets); Object[][] result = new Object[metaList.size()][]; for (int i = 0; i < metaList.size(); ++i) { ImageDatasetMetadata meta = metaList.get(i); ImageDatasetReference imageDatasetReference = imageDatasets.get(i); Geometry plateGeometry = imageDatasetReference.getPlateGeometry(); result[i] = new Object[] { meta.getWidth(), meta.getHeight(), meta.getNumberOfTiles(), meta.getTilesRows(), meta.getTilesCols(), plateGeometry.getNumberOfRows(), plateGeometry.getNumberOfColumns() }; } return result; } /** * Lists all channels measured in experiment. *

* Matlab example: * *

     * % Get the channels of experiment MYEXP in project PROJ of space SPACE
     * channels = OpenBISScreeningML.listChannels('/SPACE/PROJ/MYEXP');
     * % How many channels do we have?
     * length(channels)
     * % What is the name of channel 1?
     * channels(1)
     * 
* * @param experiment The augmented code of the experiment to list the channels for * @return Each row contains information about one channel. Currently the only information * available is the channel name. */ public static Object[][] listChannels(String experiment) { checkLoggedIn(); final List experimentPlates = experimentToPlateMap.get(experiment); if (experimentPlates == null) { throw new RuntimeException("No experiment with that code found."); } if (experimentPlates.isEmpty()) { return new Object[0][]; } final List imageDatasets = openbis.listRawImageDatasets(experimentPlates); if (imageDatasets.isEmpty()) { return new Object[0][]; } return extractChannels(imageDatasets.get(0)); } private static List getChannelCodes(final List meta) { return meta.get(0).getChannelCodes(); } /** * Lists all features computed for experiment. *

* Matlab example: * *

     * % Get the features of experiment MYEXP in project PROJ of space SPACE
     * features = OpenBISScreeningML.listFeatures('/SPACE/PROJ/MYEXP');
     * % How many features do we have?
     * length(features)
     * % What is the name of features 1?
     * features(1)
     * 
* * @param experiment The augmented code of the experiment to list the features for * @return Each row contains information about one feature. Currently the only information * available is the feature name. */ public static Object[][] listFeatures(String experiment) { checkLoggedIn(); final List experimentPlates = experimentToPlateMap.get(experiment); if (experimentPlates == null) { throw new RuntimeException("No experiment with that code found."); } if (experimentPlates.isEmpty()) { return new Object[0][]; } final List featureDatasets = openbis.listFeatureVectorDatasets(experimentPlates); if (featureDatasets.isEmpty()) { return new Object[0][]; } final List features = listAvailableFeatureCodes(featureDatasets); Object[][] result = new Object[features.size()][1]; for (int i = 0; i < result.length; ++i) { result[i][0] = features.get(i); } return result; } @SuppressWarnings("deprecation") private static List listAvailableFeatureCodes( final List featureDatasets) { return openbis.listAvailableFeatureNames(Arrays.asList(featureDatasets.get(0))); } // // Data Sets // /** * Loads data sets for specified plate code. For each data set the path to the root of the data * set is returned. If it is possible the path points directly into the data set store. No data * is copied. Otherwise the data is retrieved from the data store server.
* If the same dataset is loaded for the second time in one session it will be immediately * returned from the local cache. *

* Matlab example: * *

     * % Load all data sets of plate P005 in space SPACE
     * dsinfo = OpenBISScreeningML.loadDataSets('/SPACE/P005')
     * % Get the data set codes
     * dsinfo(:,1)
     * % Get root path of first data set (assuming there is at least one)
     * dsginfo(1,2)
     * 
* * @param augmentedPlateCode The augmented plate code. * @param datasetTypeCodePattern only datasets of the type which matche the specified pattern * will be returned. To fetch all datasets specify ".*". * @param overrideStoreRootPathOrNull A path, in the context of the local file system mounts, to * the DSS' store root. If null, paths are returned in the context of the DSS' file * system mounts. * @return Each row contains information about one data set: *

* { data set code, data set root path } */ public static Object[][] loadDataSets(String augmentedPlateCode, String datasetTypeCodePattern, String overrideStoreRootPathOrNull) { checkLoggedIn(); Plate plateIdentifier = getPlate(augmentedPlateCode); List dataSets = openbis.getDataSets(plateIdentifier, datasetTypeCodePattern); Object[][] result = new Object[dataSets.size()][]; try { for (int i = 0; i < dataSets.size(); i++) { IDataSetDss dataSet = dataSets.get(i); String code = dataSet.getCode(); File file = new File(dataSetsDir, code); if (file.exists() == false) { file = dataSet.getLinkOrCopyOfContents(overrideStoreRootPathOrNull, dataSetsDir); } result[i] = new Object[] { code, file.getPath() }; } return result; } catch (Exception ex) { throw new RuntimeException("Loading data sets for plate '" + augmentedPlateCode + "' failed: " + ex, ex); } } /** * Uploads specified data set for specified plate. The data set code will be returned. *

* Matlab example: * *

     * % Upload data set /path/to/my-data-set with properties DESCRIPTION and NUMBER for 
     * % plate P005 in space SPACE
     * properties = {'DESCRIPTION' 'hello example'; 'NUMBER' 3.14}
     * datasetcode = OpenBISScreeningML.uploadDataSet('/SPACE/P005', '/path/to/my-data-set', 'HCS_IMAGE', properties)
     * 
* * @param augmentedPlateCode The augmented plate code. * @param dataSetFilePath Path to the data set file/folder to be uploaded. * @param dataSetType Data set type. * @param dataSetProperties A two dimensional array where the first column contains the property * codes and the second column the corresponding property values. */ public static Object uploadDataSet(String augmentedPlateCode, String dataSetFilePath, String dataSetType, Object[][] dataSetProperties) { checkLoggedIn(); Plate plateIdentifier = getPlate(augmentedPlateCode); File dataSetFile = new File(dataSetFilePath); if (dataSetFile.exists() == false) { throw new RuntimeException("Unknown data set file path '" + dataSetFilePath + "'."); } try { Map map = createMap(dataSetProperties); IDataSetDss dataSet = openbis.putDataSet(plateIdentifier, dataSetFile, new NewDataSetMetadataDTO( dataSetType, map)); return dataSet.getCode(); } catch (Exception ex) { throw new RuntimeException("Couldn't upload data set for plate '" + augmentedPlateCode + "'.", ex); } } private static Map createMap(Object[][] properties) { Map map = new HashMap(); for (Object[] objects : properties) { if (objects.length == 2) { Object value = objects[1]; map.put(objects[0].toString(), value == null ? null : value.toString()); } } return map; } // // Images // /** * Loads the TIFF images for the given well location, all tiles and all channels and stores them * in temporary files. The temporary files will be removed automatically when the Java Virtual * Machine exits. *

* Matlab example: * *

     * % Load the images for all channels of well B10 of plate P005 in space SPACE
     * imginfo = OpenBISScreeningML.loadImages('/SPACE/P005', 2, 10)
     * % Get the plate-well descriptions of all locations
     * imginfo(2,:,3)
     * % Show the third image (assuming there are at least three images)
     * imtool(imginfo(1,3))
     * 
* * @param plate The augmented plate code * @param row The row in the plate to get the images for * @param col The column in the plate to get the images for * @return { names of TIFF files, image annotation } *

* Each of names of TIFF files and image annotation is a * vector of length of the number of images. *

* image annotation contains * { channel name, tile number, plate well description, * plate augmented code, plate perm id, plate space code, plate code, row, column, * experiment augmented code, experiment perm id, experiment space code, * experiment project code, experiment code, data set code } */ public static Object[][][] loadImages(String plate, int row, int col) { return loadImages(plate, row, col, (String[]) null); } /** * Loads the TIFF images for the given well location, tile number, and all channels and stores * them in temporary files. The temporary files will be removed automatically when the Java * Virtual Machine exits. *

* Matlab example: * *

     * % Load the images for all channels of well B10 and tile 3 of plate P005 in space SPACE
     * imginfo = OpenBISScreeningML.loadImages('/SPACE/P005', 2, 10, 3)
     * % Get the plate-well descriptions of all locations
     * imginfo(2,:,3)
     * % Show the third image (assuming there are at least three images)
     * imtool(imginfo(1,3))
     * 
* * @param plate The augmented plate code * @param row The row in the plate to get the images for * @param col The column in the plate to get the images for * @param tile The tile number. Starts with 0. * @return { names of TIFF files, image annotation } *

* Each of names of TIFF files and image annotation is a * vector of length of the number of images. *

* image annotation contains * { channel name, tile number, plate well description, * plate augmented code, plate perm id, plate space code, plate code, row, column, * experiment augmented code, experiment perm id, experiment space code, * experiment project code, experiment code, data set code } */ public static Object[][][] loadImages(String plate, int row, int col, int tile) { return loadImages(plate, row, col, tile, (String[]) null); } /** * Loads the TIFF images for the given well location, list of channels, and all tiles and stores * them in temporary files. The temporary files will be removed automatically when the Java * Virtual Machine exits. *

* Matlab example: * *

     * % Load the images for channel DAPI of well H10 of plate P005 in space SPACE
     * imginfo=OpenBISScreeningML.loadImages('/SPACE/P005', 8, 10, 'DAPI')
     * % Get the channel names and tile numbers of all locations
     * imginfo(2,:,1:2)
     * % Show the second image (assuming there are at least two images)
     * imtool(imginfo(1,2))
     * 
* * @param plate The augmented plate code * @param row The row in the plate to get the images for * @param col The column in the plate to get the images for * @param channels The names of the channels to get the images for * @return { names of TIFF files, image annotation } *

* Each of names of TIFF files and image annotation is a * vector of length of the number of images. *

* image annotation contains * { channel name, tile number, plate well description, * plate augmented code, plate perm id, plate space code, plate code, row, column, * experiment augmented code, experiment perm id, experiment space code, * experiment project code, experiment code, data set code } */ public static Object[][][] loadImages(String plate, int row, int col, String[] channels) { return loadRawImages(plate, row, col, channels, createAllTilesIterator()); } /** * Has the same effect as {@link #loadImages(String, int, int, String[])}, but instead of * loading raw images loads their segmentation results if available. * * @param objectNames The names of the segmentation objects to get the images for */ public static Object[][][] loadSegmentationImages(String plate, int row, int col, String[] objectNames) { return loadSegmentationImages(plate, row, col, objectNames, createAllTilesIterator()); } private static ITileNumberIterable createAllTilesIterator() { return new ITileNumberIterable() { private int maximumNumberOfTiles; public void setMaximumNumberOfTiles(int numberOfTiles) { this.maximumNumberOfTiles = numberOfTiles; } public int getMaximumNumberOfTiles() { return maximumNumberOfTiles; } public Iterator iterator() { return new Iterator() { private int index; public boolean hasNext() { return index < maximumNumberOfTiles; } public Integer next() { return index++; } public void remove() { throw new UnsupportedOperationException(); } }; } }; } /** * Loads the TIFF images for the given well location, tile number, and list of channels and * stores them in temporary files. The temporary files will be removed automatically when the * Java Virtual Machine exits. *

* Matlab example: * *

     * % Load the images for channel DAPI of well H10 and tile 3 of plate P005 in space SPACE
     * imginfo=OpenBISScreeningML.loadImages('/SPACE/P005', 8, 10, 3, 'DAPI')
     * % Get the channel names of all locations
     * imginfo(2,:,1)
     * % Show the second image (assuming there are at least two images)
     * imtool(imginfo(1,2))
     * 
* * @param plate The augmented plate code * @param row The row in the plate to get the images for * @param col The column in the plate to get the images for * @param tile The tile number. Starts with 0. * @param channels The names of the channels to get the images for * @return { names of TIFF files, image annotation } *

* Each of names of TIFF files and image annotation is a * vector of length of the number of images. *

* image annotation contains * { channel name, tile number, plate well description, * plate augmented code, plate perm id, plate space code, plate code, row, column, * experiment augmented code, experiment perm id, experiment space code, * experiment project code, experiment code, data set code } */ public static Object[][][] loadImages(String plate, int row, int col, final int tile, String[] channels) { return loadRawImages(plate, row, col, channels, createSingleTileIterator(tile)); } /** * Has the same effect as {@link #loadImages(String, int, int, int, String[])}, but instead of * loading raw images loads their segmentation results if available. * * @param objectNames The names of the segmentation objects to get the images for */ public static Object[][][] loadSegmentationImages(String plate, int row, int col, final int tile, String[] objectNames) { return loadSegmentationImages(plate, row, col, objectNames, createSingleTileIterator(tile)); } private static ITileNumberIterable createSingleTileIterator(final int tile) { return new ITileNumberIterable() { public void setMaximumNumberOfTiles(int numberOfTiles) { if (tile >= numberOfTiles) { throw new IllegalArgumentException("Tile number " + tile + " is not less than number of tiles " + numberOfTiles + "."); } } public int getMaximumNumberOfTiles() { return 1; } public Iterator iterator() { return new Iterator() { private boolean delivered; public boolean hasNext() { return delivered == false; } public Integer next() { delivered = true; return tile; } public void remove() { throw new UnsupportedOperationException(); } }; } }; } /** * Lists all segmentation objects for the plate. *

* Matlab example: * *

     * % Get the segmentation objects of plate P005 in space SPACE.
     * segmentationObjects = OpenBISScreeningML.listSegmentationObjects('/SPACE/P005');
     * % How many segmentation objects do we have?
     * length(segmentationObjects)
     * % What is the name of segmentation objects 1?
     * segmentationObjects(1)
     * 
* * @param plate augmented code of the plate * @return Each row contains information about one segmentation object. Currently the only * information available is the segmentation object name. */ public static Object[][] listSegmentationObjects(String plate) { checkLoggedIn(); Plate plateId = getPlate(plate); final List imageDatasets = listSegmentationImageDatasets(plateId); if (imageDatasets.isEmpty()) { return new Object[0][]; } return extractChannels(imageDatasets.get(0)); } private static Object[][] extractChannels(ImageDatasetReference imageDatasetReference) { final List meta = openbis.listImageMetadata(Arrays.asList(imageDatasetReference)); if (meta.isEmpty()) { return new Object[0][]; } final List channels = getChannelCodes(meta); Object[][] result = new Object[channels.size()][1]; for (int i = 0; i < result.length; ++i) { result[i][0] = channels.get(i); } return result; } private static Object[][][] loadRawImages(String plate, int row, int col, String[] channels, ITileNumberIterable tileNumberIterable) { checkLoggedIn(); final Plate plateId = getPlate(plate); final List imageDatasets = listRawImageDatasets(plateId); return loadImages(plateId, imageDatasets, row, col, channels, tileNumberIterable); } private static Object[][][] loadSegmentationImages(String plate, int row, int col, String[] channels, ITileNumberIterable tileNumberIterable) { checkLoggedIn(); final Plate plateId = getPlate(plate); final List imageDatasets = listSegmentationImageDatasets(plateId); return loadImages(plateId, imageDatasets, row, col, channels, tileNumberIterable); } private static Object[][][] loadImages(Plate plate, List imageDatasets, int row, int col, String[] channels, ITileNumberIterable tileNumberIterable) { final List meta = openbis.listImageMetadata(imageDatasets); if (meta.isEmpty()) { return new Object[][][] { new Object[0][], new Object[0][] }; } final List imageChannels; if (channels == null || channels.length == 0) { imageChannels = getChannelCodes(meta); } else { imageChannels = Arrays.asList(channels); } final List imageReferencesAndFiles = new ArrayList(imageDatasets.size()); final Object[][][] result = new Object[2][][]; tileNumberIterable.setMaximumNumberOfTiles(meta.get(0).getNumberOfTiles()); int numberOfTiles = tileNumberIterable.getMaximumNumberOfTiles(); result[0] = new Object[imageDatasets.size() * imageChannels.size() * numberOfTiles][1]; result[1] = new Object[imageDatasets.size() * imageChannels.size() * numberOfTiles][15]; int dsIdx = 0; int resultIdx = 0; for (ImageDatasetReference ds : imageDatasets) { for (String channel : imageChannels) { for (Integer tile : tileNumberIterable) { final PlateImageReference ref = new PlateImageReference(row, col, tile, channel, ds); final File imageFile = createImageFileName(plate, ref); imageReferencesAndFiles.add(new ImageReferenceAndFile(ref, imageFile)); result[0][resultIdx][0] = imageFile.getPath(); PlateIdentifier plateIdentifier = ds.getPlate(); ExperimentIdentifier expIdentifier = ds.getExperimentIdentifier(); final Object[] annotations = new Object[] { channel, tile, createPlateWellDescription(plateIdentifier, row, col), plateIdentifier.getAugmentedCode(), plateIdentifier.getPermId(), plateIdentifier.tryGetSpaceCode(), plateIdentifier.getPlateCode(), row, col, expIdentifier.getAugmentedCode(), expIdentifier.getPermId(), expIdentifier.getSpaceCode(), expIdentifier.getProjectCode(), expIdentifier.getExperimentCode(), ds.getPermId(), }; System.arraycopy(annotations, 0, result[1][resultIdx], 0, annotations.length); resultIdx++; } } dsIdx++; } try { loadImages(imageReferencesAndFiles); } catch (IOException ex) { throw new RuntimeException(ex); } return result; } private static List listRawImageDatasets(final Plate plateId) { return openbis.listRawImageDatasets(Arrays.asList(plateId)); } private static List listSegmentationImageDatasets(final Plate plateId) { return openbis.listSegmentationImageDatasets(Arrays.asList(plateId)); } /** * Saves images for a given list of image references (given by data set code, well position, * channel and tile) and files. * * @throws IOException when reading images from the server or writing them to the files fails */ private static void loadImages(List imageReferencesAndFiles) throws IOException { List imageReferences = new ArrayList(); final Map imageRefToFileMap = new HashMap(); for (ImageReferenceAndFile imageReferenceAndFile : imageReferencesAndFiles) { PlateImageReference imageReference = imageReferenceAndFile.getImageReference(); File file = loadedImages.get(imageReference); if (file == null) { imageReferences.add(imageReference); imageRefToFileMap.put(imageReference, imageReferenceAndFile); } } try { openbis.loadImages(imageReferences, new IImageOutputStreamProvider() { public OutputStream getOutputStream(PlateImageReference imageReference) throws IOException { return imageRefToFileMap.get(imageReference).open(); } }, false); } finally { Collection values = imageRefToFileMap.values(); for (ImageReferenceAndFile imageReferenceAndFile : values) { imageReferenceAndFile.close(); PlateImageReference imageReference = imageReferenceAndFile.getImageReference(); loadedImages.put(imageReference, imageReferenceAndFile.getImageFile()); } } } private static File createImageFileName(Plate plate, PlateImageReference image) { final WellPosition well = image.getWellPosition(); File imageDir = new File(temporarySessionDir, "images"); imageDir.mkdirs(); final File f = new File(imageDir, "img_" + plate.getPlateCode() + "_" + image.getDatasetCode() + "_row" + well.getWellRow() + "_col" + well.getWellColumn() + "_" + image.getChannel() + "_tile" + image.getTile() + ".tiff"); f.deleteOnExit(); return f; } // // Feature matrix // /** * Returns the feature matrix of all features for all locations in experiment (a * location is one well position in one feature vector data set) connected to gene in * [0], location annotations in [1] and feature annotation in * [2]. *

* One row in the matrix corresponds to one location (i.e. one well and one feature vector * dataset), one column corresponds to one feature. *

* Matlab example: * *

     * % Get feature matrix for experiment /SPACE/PROJ/MYEXP for locations connected to GENENAME
     * fmatrix = OpenBISScreeningML.getFeatureMatrix('/SPACE/PROJ/MYEXP', 'GENENAME');
     * % Get the feature vector for the second location (assuming there are at least two locations)
     * loc2 = fmatrix(1,2,:)
     * % Get the values of the fifth feature for all locations (assuming there are at least 5 features)
     * feature5 = fmatrix(1,:,5)
     * % What are the features?
     * featureNames = fmatrix(3,:)
     * % Get the plate-well descriptions of the locations
     * locationDescriptions = fmatrix(2,:,1)
     * 
* * @param experiment The augmented experiment code * @param gene The gene code (stored as material code in openBIS, usually it is gene id) * @return { feature matrix, annotations per location, feature names } where * annotations per location contain: *

* { plate well description, plate augmented code, plate perm id, * plate space code, plate code, row, column, experiment augmented code, experiment perm * id, experiment space code, experiment project code, experiment code, data set code } */ public static Object[][][] getFeatureMatrix(String experiment, String gene) { return getFeatureMatrix(experiment, gene, (String[]) null); } /** * Returns the feature matrix of the specified features for all locations in * experiment (a location is one well position in one feature vector data set) in * experiment connected to gene in [0], location annotations * in [1] and feature annotation in [2]. *

* One row in the matrix corresponds to one location (i.e. one well and one feature vector * dataset), one column corresponds to one feature. *

* Matlab example: * *

     * % Get feature matrix for features FEATURE1, FEATURE2 and FEATURE for 
     * % experiment /SPACE/PROJ/MYEXP for locations connected to GENENAME
     * fmatrix = OpenBISScreeningML.getFeatureMatrix('/SPACE/PROJ/MYEXP', 'GENENAME', ('FEATURE1','FEATURE2','FEATURE3'));
     * % Get the feature vector for the second location (assuming there are at least two locations)
     * loc2 = fmatrix(1,2,:)
     * % Get the values of the fourth feature for all locations (assuming there are at least 4 features)
     * feature5 = fmatrix(1,:,4)
     * % What are the features?
     * featureNames = fmatrix(3,:)
     * % Get the plate-well descriptions of the locations
     * locationDescriptions = fmatrix(2,:,1)
     * 
* * @param experiment The augmented experiment code * @param gene The gene code (stored as material code in openBIS, usually it is gene id) * @param features The names of the features to contain the feature matrix * @return { feature matrix, annotations per location, feature names } where * annotations per location contain: *

* { plate well description, plate augmented code, plate perm id, * plate space code, plate code, row, column, experiment augmented code, experiment perm * id, experiment space code, experiment project code, experiment code, data set code } */ public static Object[][][] getFeatureMatrix(String experiment, String gene, String[] features) { checkLoggedIn(); final ExperimentIdentifier experimentId = experimentCodeToExperimentMap.get(experiment); if (experimentId == null) { throw new RuntimeException("No experiment with that code found."); } final List experimentPlates = experimentToPlateMap.get(experiment); if (experimentPlates == null || experimentPlates.isEmpty()) { return new Object[][][] { new Object[0][], new Object[0][], new Object[0][] }; } final List featureVectors = openbis.loadFeaturesForPlateWells(experimentId, new MaterialIdentifier( MaterialTypeIdentifier.GENE, gene), (features == null) ? null : Arrays.asList(features)); final List featureNameList = featureVectors.get(featureVectors.size() - 1).getFeatureNames(); final Object[][][] result = new Object[3][][]; if (featureVectors.isEmpty()) { return result; } result[0] = new Object[featureVectors.size()][featureNameList.size()]; result[1] = new Object[featureVectors.size()][13]; int resultIdx = 0; for (FeatureVectorWithDescription f : featureVectors) { arraycopy(f.getValues(), result[0][resultIdx]); final Object[] annotations = new Object[] { createPlateWellDescription(f), f.getDatasetWellReference().getPlate().getAugmentedCode(), f.getDatasetWellReference().getPlate().getPermId(), f.getDatasetWellReference().getPlate().tryGetSpaceCode(), f.getDatasetWellReference().getPlate().getPlateCode(), f.getWellPosition().getWellRow(), f.getWellPosition().getWellColumn(), f.getDatasetWellReference().getExperimentIdentifier() .getAugmentedCode(), f.getDatasetWellReference().getExperimentIdentifier().getPermId(), f.getDatasetWellReference().getExperimentIdentifier() .getSpaceCode(), f.getDatasetWellReference().getExperimentIdentifier() .getProjectCode(), f.getDatasetWellReference().getExperimentIdentifier() .getExperimentCode(), f.getDatasetWellReference().getDatasetCode(), }; System.arraycopy(annotations, 0, result[1][resultIdx], 0, annotations.length); resultIdx++; } result[2] = new Object[featureNameList.size()][1]; for (int i = 0; i < featureNameList.size(); ++i) { result[2][i][0] = featureNameList.get(i); } return result; } /** * Returns the feature matrix of all features for all locations (a location is one well position * in one feature vector data set) connected to gene in [0], location * annotations in [1] and feature annotation in [2]. *

* One row in the matrix corresponds to one location (i.e. one well and one feature vector * dataset), one column corresponds to one feature. *

* Matlab example: * *

     * % Get feature matrix for GENENAME
     * fmatrix = OpenBISScreeningML.getFeatureMatrix('GENENAME');
     * % Get the feature vector for the second location (assuming there are at least two locations)
     * loc2 = fmatrix(1,2,:)
     * % Get the values of the fifth feature for all locations (assuming there are at least 5 features)
     * feature5 = fmatrix(1,:,5)
     * % What are the features?
     * featureNames = fmatrix(3,:)
     * % Get the plate-well descriptions of the locations
     * locationDescriptions = fmatrix(2,:,1)
     * 
* * @param gene The gene code (stored as material code in openBIS, usually it is gene id) * @return { feature matrix, annotations per location, feature names } where * annotations per location contain: *

* { plate well description, plate augmented code, plate perm id, * plate space code, plate code, row, column, experiment augmented code, experiment perm * id, experiment space code, experiment project code, experiment code, data set code } */ public static Object[][][] getFeatureMatrix(String gene) { return getFeatureMatrix(gene, (String[]) null); } /** * Returns the feature matrix of the specified features for all locations (a location is one * well position in one feature vector data set) in experiment connected to * gene in [0], location annotations in [1] and feature * annotation in [2]. *

* One row in the matrix corresponds to one location (i.e. one well and one feature vector * dataset), one column corresponds to one feature. *

* Matlab example: * *

     * % Get feature matrix for features FEATURE1, FEATURE2 and FEATURE for GENENAME
     * fmatrix = OpenBISScreeningML.getFeatureMatrix('GENENAME', ('FEATURE1','FEATURE2','FEATURE3'));
     * % Get the feature vector for the second location (assuming there are at least two locations)
     * loc2 = fmatrix(1,2,:)
     * % Get the values of the second feature ('FEATURE2' here) for all locations
     * feature2 = fmatrix(1,:,2)
     * % What are the features?
     * featureNames = fmatrix(3,:)
     * % Get the plate-well descriptions of the locations
     * locationDescriptions = fmatrix(2,:,1)
     * 
* * @param gene The gene code (stored as material code in openBIS, usually it is gene id) * @param features The names of the features to contain the feature matrix * @return { feature matrix, annotations per location, feature names } where * annotations per location contain: *

* { plate well description, plate augmented code, plate perm id, * plate space code, plate code, row, column, experiment augmented code, experiment perm * id, experiment space code, experiment project code, experiment code, data set code } */ public static Object[][][] getFeatureMatrix(String gene, String[] features) { checkLoggedIn(); final List featureVectors = openbis.loadFeaturesForPlateWells(new MaterialIdentifier( MaterialTypeIdentifier.GENE, gene), (features == null) ? null : Arrays.asList(features)); final List featureNameList = featureVectors.get(featureVectors.size() - 1).getFeatureNames(); final Object[][][] result = new Object[3][][]; if (featureVectors.isEmpty()) { return result; } result[0] = new Object[featureVectors.size()][featureNameList.size()]; result[1] = new Object[featureVectors.size()][13]; int resultIdx = 0; for (FeatureVectorWithDescription f : featureVectors) { arraycopy(f.getValues(), result[0][resultIdx]); final Object[] annotations = new Object[] { createPlateWellDescription(f), f.getDatasetWellReference().getPlate().getAugmentedCode(), f.getDatasetWellReference().getPlate().getPermId(), f.getDatasetWellReference().getPlate().tryGetSpaceCode(), f.getDatasetWellReference().getPlate().getPlateCode(), f.getWellPosition().getWellRow(), f.getWellPosition().getWellColumn(), f.getDatasetWellReference().getExperimentIdentifier() .getAugmentedCode(), f.getDatasetWellReference().getExperimentIdentifier().getPermId(), f.getDatasetWellReference().getExperimentIdentifier() .getSpaceCode(), f.getDatasetWellReference().getExperimentIdentifier() .getProjectCode(), f.getDatasetWellReference().getExperimentIdentifier() .getExperimentCode(), f.getDatasetWellReference().getDatasetCode(), }; System.arraycopy(annotations, 0, result[1][resultIdx], 0, annotations.length); resultIdx++; } result[2] = new Object[featureNameList.size()][1]; for (int i = 0; i < featureNameList.size(); ++i) { result[2][i][0] = featureNameList.get(i); } return result; } /** * Returns the feature matrix of all available features for all locations (a location is one * well position in one feature vector data set) of all feature vector data sets of the given * plate in [0], location annotations in [1] and feature * annotation in [2]. *

* One row in the matrix corresponds to one location (i.e. one well and one feature vector * dataset), one column corresponds to one feature. *

* Matlab example: * *

     * % Get feature matrix for PLATECODE
     * fmatrix = OpenBISScreeningML.getFeatureMatrixForPlate('PLATECODE');
     * % Get the feature vector for the second location (assuming there are at least two locations)
     * loc2 = fmatrix(1,2,:)
     * % Get the values of the fourth feature for all locations (assuming there are at least 4 features)
     * feature5 = fmatrix(1,:,4)
     * % What are the features?
     * featureNames = fmatrix(3,:)
     * % Get the plate-well descriptions of the locations
     * locationDescriptions = fmatrix(2,:,1)
     * 
* * @param plate augmented code of the plate for which features should be loaded * @return { feature matrix, annotations per location, feature names } where * annotations per location contain: *

* { plate well description, plate augmented code, plate perm id, * plate space code, plate code, row, column, experiment augmented code, experiment perm * id, experiment space code, experiment project code, experiment code, data set code } */ public static Object[][][] getFeatureMatrixForPlate(String plate) { return getFeatureMatrixForPlate(plate, (String[]) null); } /** * Returns the feature matrix of the specified features for all locations (a location is one * well position in one feature vector data set) of all feature vector data sets of the given * plate in [0], location annotations in [1] and feature * annotation in [2]. *

* One row in the matrix corresponds to one location (i.e. one well and one feature vector * dataset), one column corresponds to one feature. *

* Matlab example: * *

     * % Get feature matrix for features FEATURE1, FEATURE2 and FEATURE for PLATECODE
     * fmatrix = OpenBISScreeningML.getFeatureMatrixForPlate('PLATECODE', ('FEATURE1','FEATURE2','FEATURE3'));
     * % Get the feature vector for the second location (assuming there are at least two locations)
     * loc2 = fmatrix(1,2,:)
     * % Get the values of the second feature for all locations
     * feature5 = fmatrix(1,:,2)
     * % What are the features?
     * featureNames = fmatrix(3,:)
     * % Get the plate-well descriptions of the locations
     * locationDescriptions = fmatrix(2,:,1)
     * 
* * @param plate augmented code of the plate for which features should be loaded * @param features The names of the features to contain the feature matrix * @return { feature matrix, annotations per location, feature names } where * annotations per location contain: *

* { plate well description, plate augmented code, plate perm id, * plate space code, plate code, row, column, experiment augmented code, experiment perm * id, experiment space code, experiment project code, experiment code, data set code } */ public static Object[][][] getFeatureMatrixForPlate(String plate, String[] features) { checkLoggedIn(); final List featureVectors = openbis.loadFeaturesForPlates( Arrays.asList(PlateIdentifier.createFromAugmentedCode(plate)), (features == null) ? null : Arrays.asList(features)); FeatureVectorDataset last = featureVectors.get(featureVectors.size() - 1); final List featureCodeList = getFeatureCodes(last); final Object[][][] result = new Object[3][][]; if (featureVectors.isEmpty()) { return result; } int numberOfRows = 0; for (FeatureVectorDataset fvds : featureVectors) { numberOfRows += fvds.getFeatureVectors().size(); } result[0] = new Object[numberOfRows][featureCodeList.size()]; result[1] = new Object[numberOfRows][13]; int resultIdx = 0; for (FeatureVectorDataset fvds : featureVectors) { final FeatureVectorDatasetReference datasetRef = fvds.getDataset(); for (FeatureVector f : fvds.getFeatureVectors()) { arraycopy(f.getValues(), result[0][resultIdx]); final Object[] annotations = new Object[] { createPlateWellDescription(datasetRef.getPlate(), f), datasetRef.getPlate().getAugmentedCode(), datasetRef.getPlate().getPermId(), datasetRef.getPlate().tryGetSpaceCode(), datasetRef.getPlate().getPlateCode(), f.getWellPosition().getWellRow(), f.getWellPosition().getWellColumn(), datasetRef.getExperimentIdentifier().getAugmentedCode(), datasetRef.getExperimentIdentifier().getPermId(), datasetRef.getExperimentIdentifier().getSpaceCode(), datasetRef.getExperimentIdentifier().getProjectCode(), datasetRef.getExperimentIdentifier().getExperimentCode(), datasetRef.getDatasetCode(), }; System.arraycopy(annotations, 0, result[1][resultIdx], 0, annotations.length); resultIdx++; } } result[2] = new Object[featureCodeList.size()][1]; for (int i = 0; i < featureCodeList.size(); ++i) { result[2][i][0] = featureCodeList.get(i); } return result; } @SuppressWarnings("deprecation") private static List getFeatureCodes(FeatureVectorDataset last) { return last.getFeatureNames(); } /** * Returns the gene mapping for the given plateCodes in [0] and location * annotations in [1]. *

* One row in the matrix corresponds to one well. *

* Matlab example: * *

     * % Get feature matrix for features FEATURE1, FEATURE2 and FEATURE for PLATECODE
     * genes = getGeneMappingForPlate('PLATECODE');
     * % Get the plate well location description of the 10th wells
     * loc2 = genes(2,10,1)
     * % Get the gene ids that are in the 10th well
     * geneIds = genes(1,10,:)
     * 
* * @param platesCodes The augmented codes of the plates to get the mapping for * @return { gene ids, annotations per well } where gene ids can be 0, * 1 or more gene ids. annotations per location contain: *

* { plate well description, plate augmented code, plate perm id, * plate space code, plate code, row, column } */ public static Object[][][] getGeneMappingForPlates(String[] platesCodes) { checkLoggedIn(); final List mappingList = openbis.listPlateMaterialMapping(toPlates(platesCodes), MaterialTypeIdentifier.GENE); int size = 0; for (PlateWellMaterialMapping mapping : mappingList) { size += mapping.getPlateGeometry().getNumberOfRows() * mapping.getPlateGeometry().getNumberOfColumns(); } final Object[][][] result = new Object[2][size][]; int resultIdx = 0; for (PlateWellMaterialMapping mapping : mappingList) { for (int row = 1; row <= mapping.getPlateGeometry().getNumberOfRows(); ++row) { for (int col = 1; col <= mapping.getPlateGeometry().getNumberOfColumns(); ++col) { final List genes = mapping.getMaterialsForWell(row, col); result[0][resultIdx] = new Object[genes.size()]; for (int i = 0; i < genes.size(); ++i) { result[0][resultIdx][i] = genes.get(i).getMaterialCode(); } final PlateIdentifier plate = mapping.getPlateIdentifier(); result[1][resultIdx] = new Object[] { createPlateWellDescription(plate, row, col), plate.getAugmentedCode(), plate.getPermId(), plate.tryGetSpaceCode(), plate.getPlateCode(), row, col, }; ++resultIdx; } } } return result; } // // Helper methods // private static List toPlates(String[] augmentedPlateCodes) { final List result = new ArrayList(augmentedPlateCodes.length); for (String plateCode : augmentedPlateCodes) { result.add(PlateIdentifier.createFromAugmentedCode(plateCode)); } return result; } private static Plate getPlate(String augmentedPlateCode) { Plate plateIdentifier = plateCodeToPlateMap.get(augmentedPlateCode); if (plateIdentifier == null) { throw new RuntimeException("No plate with that code '" + augmentedPlateCode + "' found."); } return plateIdentifier; } private static void arraycopy(double[] src, Object[] dest) { for (int i = 0; i < dest.length; ++i) { dest[i] = src[i]; } } private static String createPlateWellDescription(FeatureVectorWithDescription f) { return createPlateWellDescription(f.getDatasetWellReference().getPlate(), f .getWellPosition().getWellRow(), f.getWellPosition().getWellColumn()); } private static String createPlateWellDescription(PlateIdentifier p, FeatureVector f) { return createPlateWellDescription(p, f.getWellPosition().getWellRow(), f.getWellPosition() .getWellColumn()); } private static String createPlateWellDescription(PlateIdentifier p, int row, int col) { return p.getPlateCode() + ":" + translateRowNumberIntoLetterCode(row) + col; } /** * Translates a row number into letter code. Thus, 1 -> A, 2 -> B, 26 -> Z, 27 -> AA, 28 -> AB, * etc. */ private static String translateRowNumberIntoLetterCode(int rowNumber) { int rowIndex = rowNumber - 1; String code = ""; while (rowIndex >= 0) { code = (char) (rowIndex % 26 + 'A') + code; rowIndex = rowIndex / 26 - 1; } return code; } private static void checkLoggedIn() { if (openbis == null) { if (Login.OPENBIS_TOKEN_FILE.exists()) { BufferedReader br = null; try { br = new BufferedReader(new FileReader(Login.OPENBIS_TOKEN_FILE)); final String token = br.readLine(); br.close(); br = new BufferedReader(new FileReader(Login.OPENBIS_SERVER_URL_FILE)); final String serverUrl = br.readLine(); br.close(); br = null; IScreeningOpenbisServiceFacade facade = facadeFactory.tryToCreate(token, serverUrl); if (facade == null) { throw new RuntimeException("Login failed."); } init(facade); } catch (IOException ex) { if (openbis == null) { throw new RuntimeException("Login failed.", ex); } } finally { if (br != null) { try { br.close(); } catch (IOException ex) { // Silence this. } } } } if (openbis == null) { throw new RuntimeException("Not logged in."); } } } }